Poor Sensitivity of the MALDI Biotyper® MBT Subtyping Module for Detection of Klebsiella pneumoniae Carbapenemase (KPC) in Klebsiella Species

Fecha de publicación: 01/01/2023 Fecha Ahead of Print: 20/09/2023

Autores organización

Jinnethe Cristina Reyes Manrique

Autor

Autores

Cuello L
Alvarez Otero J
Greenwood-Quaintance KE
Chen L
Hanson B
Komarow L
Ge L
Lancaster ZD
Gordy GG
Schuetz AN
Patel R

Grupos de investigación

Unidad de Genética y Resistencia Antimicrobiana UGRA

Resumen

Rapid detection of Klebsiella pneumoniae carbapenemase (KPC) in the Klebsiella species is desirable. The MALDI Biotyper® MBT Subtyping Module (Bruker Daltonics) uses an algorithm that detects a peak at ~11,109 m/z corresponding to a protein encoded by the p019 gene to detect KPC simultaneously with organism identification by a matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-ToF MS). Here, the subtyping module was evaluated using 795 clinical Klebsiella isolates, with whole genome sequences used to assess for blaKPC and p019. For the isolates identified as KPC positive by sequencing, the overall sensitivity of the MALDI-ToF MS subtyping module was 239/574 (42%) with 100% specificity. For the isolates harboring p019, the subtyping module showed a sensitivity of 97% (239/246) and a specificity of 100%. The subtyping module had poor sensitivity for the detection of blaKPC-positive Klebsiella isolates, albeit exhibiting excellent specificity. The poor sensitivity was a result of p019 being present in only 43% of the blaKPC-positive Klebsiella isolates. © 2023 by the authors.

Datos de la publicación

ISSN/ISSNe:: 2079-6382, 2079-6382
Tipo:: Article
Páginas:: -
DOI:: 10.3390/antibiotics12091465
Enlace a otro recurso:: www.scopus.com

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Métricas

Filiaciones

Cuello L:: Infectious Diseases Research Laboratory, Mayo Clinic, Rochester, 55905, MN, United States
Alvarez Otero J:: Infectious Diseases Research Laboratory, Mayo Clinic, Rochester, 55905, MN, United States
Greenwood-Quaintance KE:: Infectious Diseases Research Laboratory, Mayo Clinic, Rochester, 55905, MN, United States
Chen L:: Hackensack Meridian Health Center for Discovery and Innovation, Nutley, 07110, NJ, United States
Hanson B:: Department of Epidemiology, Human Genetics & Environmental Sciences, School of Public Health, The University of Texas Health Science Center at Houston, Houston, 77030, TX, United States
Reyes J:: Universidad. Molecular Genetics and Antimicrobial Resistance Unit, Universidad El Bosque, Bogotá, 110121, Colombia
Komarow L:: The Biostatistics Center, The George Washington University, Rockville, 20852, MD, United States
Ge L:: The Biostatistics Center, The George Washington University, Rockville, 20852, MD, United States
Lancaster ZD:: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, 55905, MN, United States
Gordy GG:: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, 55905, MN, United States
Schuetz AN:: Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, 55905, MN, United States
Patel R:: Infectious Diseases Research Laboratory, Mayo Clinic, Rochester, 55905, MN, United States

Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, 55905, MN, United States

Division of Public Health, Infectious Diseases and Occupational Medicine, Department of Medicine, Mayo Clinic, Rochester, 55905, MN, United States

Keywords

carbapenemase; algorithm; Article; calibration; carbapenem resistance; controlled study; diagnostic test accuracy study; Klebsiella; Klebsiella pneumoniae; mass spectrometry; matrix assisted laser desorption ionization time of flight mass spectrometry; minimum inhibitory concentration; nonhuman; proteomics; public health; sensitivity analysis; sensitivity and specificity; Staphylococcus aureus; whole genome sequencing