Differential gut microbiome in spondyloarthritis patients associated to Blastocystis colonization

Fecha de publicación: 01/01/2023 Fecha Ahead of Print: 18/08/2023

Autores organización

• 

  Liliana Morales De La Pava

  Autor

• Maria Consuelo Romero Sanchez

  Autor

• 

  Manuel Alejandro Ramos Casallas

  Autor

• 

  Javier Antonio Escobar Perez

  Autor

• Wilson Armando Bautista Molano

  Autor

• Jacqueline Chaparro Olaya

  Autor

Autores

• Nieto-Clavijo C
• Marquez-Ortiz RA
• Bello-Gualtero JM

Grupos de investigación

• Grupo de Inmunología celular y molecular Universidad El Bosque (InmuBo)

  Investigador

  Maria Consuelo Romero Sanchez

• Laboratorio de Genética Molecular Bacteriana LGMB

  

  Investigador

  Javier Antonio Escobar Perez

• Laboratorio de Parasitología Molecular

  Investigador

  Jacqueline Chaparro Olaya

Resumen

The role of Blastocystis in intestinal health is an open controversy, and little is known about the potential effect of this microorganism in autoinflammatory diseases such as spondyloarthritis (SpA). Here, we analyzed the gut microbiome of 36 SpA patients and 13 control individuals and demonstrated that the richness, diversity, and taxonomic composition between these two groups are different. We also showed that colonization by Blastocystis in control individuals increases the richness and diversity of the intestinal microbiome, whereas in SpA patients, it does not seem to have any impact. This may reflect a potential role of Blastocystis in sculpting the gut microbiome architecture in control individuals, whereas in subjects with SpA, the modulation of the microbiome may be governed by disease-dependent factors that cannot be overcome by Blastocystis. Regarding taxonomic characterization, SpA patients colonized by Blastocystis showed significant increases in the phylum Pseudomonadota, class Gammaproteobacteria, family Succinivibrionaceae, and genus Succinivibrio. Simultaneously, there were significant increases in the class Bacilli, order Lactobacillales, families Lactobacillaceae and Clostridiaceae, and genera Lactobacillus and Clostridium in non-colonized SpA patients. On the other hand, PICRUSt analysis in Blastocystis-positive SpA patients showed elevations in pathways that may enhance antioxidant capacities and alleviate intestinal inflammation, while Blastocystis-negative SpA patients showed significant changes in pathways that promote cell division/proliferation and can lead to larger changes in the gut microbiome. Our analyses lead us to believe that these changes in the gut microbiome of SpA patients may trigger protective mechanisms as an initial response to inflammation in an attempt to restore balance in the intestinal environment. © 2023, Springer Nature Limited.

© 2023. Springer Nature Limited.

Keywords

• Blastocystis; Gastrointestinal Microbiome; Humans; Inflammation; Microbiota; Spondylarthritis; Blastocystis; human; inflammation; intestine flora; microflora; spondylarthritis