Colonoscopy aspiration lavages for mucosal metataxonomic profiling of spondylarthritis-associated gastrointestinal tract alterations

Autores organización

• 

  Deisy Julieth Abril Riaño

  Autor

• 

  Javier Antonio Escobar Perez

  Autor

• Viviana Viviana Parra Izquierdo

  Autor

• Maria Consuelo Romero Sanchez

  Autor

Autores

• Marquez-Ortiz RA
• Leon M
• Florez-Sarmiento C
• Chalem P

Grupos de investigación

• Grupo de Inmunología celular y molecular Universidad El Bosque (InmuBo)

  Investigador

  Maria Consuelo Romero Sanchez

• Laboratorio de Genética Molecular Bacteriana LGMB

  

  Investigador

  Javier Antonio Escobar Perez

Resumen

The study of the GI-tract microbiota of spondylarthritis (SpA) patients has focused on the analysis of feces samples, that picture mostly the luminal microbiota. The aim of this study was to determine the contribution of mucosal and luminal microbiome to the gut dysbiosis in SpA, using colonoscopy aspiration lavages (CAL), a recent alternative for regional studies of the GI-tract. We analyzed 59 CAL (from sigmoid colon and distal ileum), and 41 feces samples, from 32 SpA patients and 7 healthy individuals, using 16S rRNA gene-targeted metataxonomic profiling. It was found high prevalence of GI-tract manifestations among SpA patients (65.3%). Metataxonomic profiling, confirmed CAL samples from the lower GI tract (colon or ileum) presented a distinctive and undifferentiated bacteriome and separate from that found in feces’ samples or in the beginning of the GI tract (oral cavity (OC)). Lower GI-tract samples and feces of SpA patients exhibited similar behavior to the microbiota of IBD group with reduced microbial richness and diversity, comparing to the healthy controls. Interestingly, it was found increase in proinflammatory taxa in SpA patients, such as Enterobacteriaceae family (mostly in the ileum), Succinivibrio spp. and Prevotella stercorea. Conversely, SpA patients presented significant decrease in the SCFA producers Coprococcus catus and Eubacterium biforme. Our data support the value of CAL samples for the regional study of GI-tract and contribute with information of potential “disruptor taxa” involved in the GI-tract associated disorders observed in SpA patients. © 2023, The Author(s).

© 2023. The Author(s).

Keywords

• Colonoscopy; Feces; Gastrointestinal Microbiome; Gastrointestinal Tract; Humans; Ileum; RNA, Ribosomal, 16S; Spondylarthritis; Therapeutic Irrigation; RNA 16S; colonoscopy; feces; gastrointestinal tract; genetics; human; ileum; intestine flora; lavage; microbiology; spondylarthritis