Porphyromonas gulae and PPAD antibodies are not related to citrullination in rheumatoid arthritis

Fecha de publicación: 01/01/2023 Fecha Ahead of Print: 01/05/2023

Autores organización

• Camilo Andres Castellar Mendoza

  Autor

• 

  Diana Marcela Castillo Perdomo

  Autor

• Leidy Lorena Chila Moreno

  Autor

• Wilson Armando Bautista Molano

  Autor

• Maria Consuelo Romero Sanchez

  Autor

Grupos de investigación

• Grupo de Inmunología celular y molecular Universidad El Bosque (InmuBo)

  Investigador

  Maria Consuelo Romero Sanchez

• Unidad de Genética y Resistencia Antimicrobiana UGRA

• Unidad de Investigación Básica Oral UIBO

  

  Investigador

  Gloria Ines Lafaurie Villamil

Resumen

Introduction: Porphyromonas gulae have the enzyme PPAD, as P. gingivalis, which is responsible for citrullination related to the pathophysiology of rheumatoid arthritis and periodontitis; this implies the presence of two species of PPAD-producing bacteria in the mouth as well as the presence of citrullinated proteins. There are no previous reports or studies investigating an association between P. gulae PPAD in rheumatoid arthritis (RA). Objective: To assess the presence of P. gulae and anti-citrullinated peptide antibodies of P. gulae PAD in patients with RA and their possible relationship with clinical activity markers. Subjects and methods: A total of 95 patients with RA and 95 controls were included. Erythrocyte sedimentation rate (ESR), C-reactive protein, anti-citrullinated protein antibodies (ACPAs) and rheumatoid factor (RF) were measured. Activity index-28 (DAS28) and SCDAI. The periodontal diagnosis was established. Presence of P. gulae and P. gingivalis. An ELISA was used to determine antibodies against citrullinated peptides of P. gulae PAD. Results: A P. gulae frequency of 15.8% was observed in the RA group and 9.5% in the control group. Higher levels of ACPA were found in the P. gulae-positive patients of the RA group, finding no significant difference, but if in patients positive for P. gingivalis with statistical significance (p = 0.0001). The frequency of anti-VDK-cit and anti-LPQ-cit9 antibodies to PPAD of P. gulae was higher in the RA group than in the control group without significant difference. No relationship was found with the clinical variables despite the presence of P. gulae and anti-citrullinated peptide antibodies of P. gulae PPAD in patients with RA Conclusions: It was not possible to establish a connection with clinical variables in RA and P. gulae; as a result, the presence of P. gingivalis continues to contribute significantly to the increase in antibodies against citrullinated proteins/peptides from exogenous sources of citrullination in RA and periodontitis. © 2023, The Author(s).

© 2023. The Author(s).

Keywords

• Anti-Citrullinated Protein Antibodies; Arthritis, Rheumatoid; Citrullination; Humans; Peptides; Periodontitis; Porphyromonas gingivalis; Porphyromonas gulae; Protein-Arginine Deiminases; cyclic citrullinated peptide antibody; peptide; protein arginine deiminase; citrullination; human; metabolism; microbiology; periodontitis; Porphyromonas gingivalis; rheumatoid arthritis