A new model for the formation of an Enterococcus faecalis endodontic biofilm with nutritional restriction

Autores organización

• Yormaris Castillo Romero

  Autor

• 

  Gloria Ines Lafaurie Villamil

  Autor

• 

  Diana Marcela Castillo Perdomo

  Autor

Autores

• Gutiérrez DM
• Ibarra-Avila H
• López M
• Orozco JC

Grupos de investigación

• Unidad de Investigación Básica Oral UIBO

  

  Investigador

  Gloria Ines Lafaurie Villamil

Resumen

An in vitro model for the formation of an Enterococcus faecalis endodontic biofilm under nutritional restriction was established, simulating clinical conditions for the evaluation of antimicrobial substances. Biofilm formation in dentin was standardized using root quarters incubated with E. faecalis ATCC 29212 at 37°C without nutritional changes. Biofilms were evaluated at 7, 14, and 30 days, counting bacterial colony-forming units using conventional culture and verified scanning electron microscopy. Bacterial viability and biovolume were determined with confocal laser microscopy. Colonization of E. faecalis and biofilm formation on the dentinal surface was confirmed after 7 and 14 days, respectively. Microorganism colonization was homogeneous over the entire root surface at each time point, without significant differences in the viability percentage and biovolume. On the contrary, a decrease in viability and an increase in biovolume were observed when the time was increased. Compared with other incubation times, 14 days was found to be the best time for the establishment of the biofilm in terms of biovolume and bacterial viability. This in vitro model for the formation of endodontic biofilm will allow future evaluation of the efficacy of antimicrobial substances with a more adequate clinical approach. © 2021 Wiley-VCH GmbH

© 2021 Wiley-VCH GmbH.

Keywords

• Anti-Bacterial Agents; Biofilms; Enterococcus faecalis; Microbial Viability; Microscopy, Confocal; Microscopy, Electron, Scanning; antiinfective agent; biofilm; confocal microscopy; Enterococcus faecalis; microbial viability; scanning electron microscopy