Activity of the Giardia intestinalis proteasome during encystation and its connection with the expression of the cyst wall protein 1 (CWP1)

Autores organización

• 

  Eliana Patricia Calvo Tapiero

  Autor

Autores

• Alvarado ME
• Chaparro-Gutiérrez JJ
• Prada LF
• Wasserman M

Grupos de investigación

• Grupo de Virología

  

  Investigador

  Jaime Eduardo Castellanos Parra

Resumen

Giardia is a parasite whose life cycle is composed of two stages: replicative trophozoites, responsible for the symptoms of the disease, and infective cysts, resistant to adverse environments outside of hosts. Proteasomes are multicatalytic peptidase complexes responsible for the specific degradation of proteins in eukaryotic cells. This study assessed the proteasome activity in the trophozoite and during encystation. Strong activation of the proteasome was observed during the differentiation of trophozoites into cysts, reaching its maximum level 24 h after the stimulus. We also found that the Giardia proteasome presents unusual characteristics related to higher eukaryotic proteasomes, making it an eventual therapeutic target. Here we tested the effects on the synthesis of a cyst wall protein by chemical inactivation of the proteasome and by overexpression or partial inhibition of the deubiquitinating protein RPN11 in transfected cells. Moreover, an analysis of the intracellular localization of RPN11 (an integral part of the proteasome regulatory particle) revealed major changes associated with the differentiation of trophozoites into cysts. This evidence further supports the important role of the proteasome in Giardia encystation. © 2021

Copyright © 2021. Published by Elsevier B.V.

Keywords

• Animals; Cysts; Giardia lamblia; Proteasome Endopeptidase Complex; Protozoan Proteins; Trophozoites; Eukaryota; Giardia; Giardia intestinalis; cyst wall protein 1; microorganism protein; proteasome; ubiquitin; unclassified drug; proteasome; protozoal protein; cell; cyst; digestive system; eukaryote; gene expression; inhibition; parasite; protein; Article; cell differentiation; controlled study; genetic transcription; Giardia intestinalis; nonhuman; parasite encystment; protein degradation; protein expression; protein localization; trophozoite; ubiquitination; animal; cyst; genetics; Giardia intestinalis