Portal de investigación
Genotyping Squamous Cell Lung Carcinoma in Colombia (Geno1.1-CLICaP)
Fecha de publicación:
Fecha Ahead of Print:
Autores organización
Autores
- Ruiz-Patiño A
- Arrieta O
- Ricaurte L
- Zatarain-Barrón ZL
- Rodriguez J
- Avila J
- Recondo G
- Barron F
- Archila P
- Sotelo C
- Bravo M
- Zamudio N
- Corrales L
- Martín C
- Rolfo C
- Viola L
- Carranza H
- Vargas C
- Otero J
- Bermudez M
- Gamez T
- Pino LE
- Rosell R
Grupos de investigación
Resumen
Background: Lung cancer is a public health problem, and squamous cell carcinoma (SCC) is the second most prevalent subtype of this neoplasm. Compared to other subtypes, including adenocarcinoma, SCC is less well understood in terms of molecular pathogenesis, limiting therapeutic options among targeted agents approved for other disease subgroups. In this study, we sought to characterize the SCC genomic profile using a validated Next Generation Sequencing (NGS) platform. Methods: The comprehensive NGS assay (TruSight Tumor 170) was used in order to target the full coding regions of 170 cancer-related genes on SCC samples. PD-L1 expression in tumor cells (TCs) was assessed using clone 22C3 (Dako). Clinical outcomes were correlated with molecular profile, including progression free survival (PFS), overall response rate (ORR), and overall survival (OS). Results: A total of 26 samples were included, median age was 67 years (r, 33–83) and 53.8% were men. Tobacco consumption was identified in all subjects (mean 34-year package). For first-line treatment 80.8% of patients received cisplatin or carboplatin plus gemcitabine. In terms of molecular profile, we identified a high prevalence of inactivating mutations in TP53 (61.5%), PIK3CA (34.6%), MLL2 (34.6%), KEAP1 (38.4%), and NOTCH1 (26.9%). PD-L1 expression ranged from negative, 1, 2–49, and =50% in 23.1, 38.5, 26.9, and 11.5%, respectively. Interestingly, the genetic alterations did not have an effect in PFS, OS or ORR in this study. However, PDL1 expression was higher among those who had mutations in TP53 (p = 0.037) and greater expression of PDL1 was related to PIK3CA alterations (p = 0.05). Conclusions: The genomic profile of SCC encompasses important genes including TP53, PIK3CA and KEAP1. TP53 mutations could be associated with PDL1 expression, generating hypothesis regarding specific treatment options. © Copyright © 2020 Cardona, Ruiz-Patiño, Arrieta, Ricaurte, Zatarain-Barrón, Rodriguez, Avila, Rojas, Recondo, Barron, Archila, Sotelo, Bravo, Zamudio, Corrales, Martín, Rolfo, Viola, Carranza, Vargas, Otero, Bermudez, Gamez, Pino and Rosell.
Copyright © 2020 Cardona, Ruiz-Patiño, Arrieta, Ricaurte, Zatarain-Barrón, Rodriguez, Avila, Rojas, Recondo, Barron, Archila, Sotelo, Bravo, Zamudio, Corrales, Martín, Rolfo, Viola, Carranza, Vargas, Otero, Bermudez, Gamez, Pino and Rosell.
Datos de la publicación
- ISSN/ISSNe:
- 2234-943X, 2234-943X
- Tipo:
- Article
- Páginas:
- 588932-588932
- Enlace a otro recurso:
- www.scopus.com
Frontiers In Oncology Frontiers Media S.A.
Citas Recibidas en Web of Science: 2
Citas Recibidas en Scopus: 4
Documentos
- No hay documentos
Filiaciones
Keywords
- buffer; carboplatin; cisplatin; cyclin D1; cyclin dependent kinase inhibitor 2A; docetaxel; epidermal growth factor receptor; gemcitabine; Notch1 receptor; phosphatase and tensin homolog; programmed death 1 ligand 1; protein p53; proteinase K; transcription factor Sox2; unclassified drug; vinorelbine tartrate; adult; aged; antigen retrieval; Article; cancer prognosis; cancer staging; centrifugation; clinical article; clinical outcome; DNA extraction; female; gene expression; genotype; high throughput sequencing; human; illumina sequencing; immunohistochemistry; male; microarray analysis; missense mutation; overall survival; prevalence; progression free survival; protein expression; quality control; RNA extraction; squamous cell carcinoma; squamous cell lung carcinoma; treatment response; whole exome sequencing
Proyectos asociados
Determinación de la huella transcipcional de mastocitos aislados de pulpa dental humana sana y con signos clínicos de inflamación
Investigador Principal: MARIA ROSA BUENAHORA TOBAR
PCI-2019-10765 . 2020
EFECTO DEL EXTRACTO DE Trametes versicolor SOBRE EL FENOTIPO Y LA FUNCIÓN DE CÉLULAS DENDRÍTICAS HUMANAS CONDICIONADAS CON MEDIOS DE CÉLULAS DE CARCINOMA ORAL
Investigador Principal: ANDRES FELIPE CARDONA MENDOZA
PCI-2019-10805 . 2020
Citar la publicación
Cardona AF,Ruiz A,Arrieta O,Ricaurte L,Zatarain ZL,Rodriguez J,Avila J,Rojas L,Recondo G,Barron F,Archila P,Sotelo C,Bravo M,Zamudio N,Corrales L,Martín C,Rolfo C,Viola L,Carranza H,Vargas C,Otero J,Bermudez M,Gamez T,Pino LE,Rosell R. Genotyping Squamous Cell Lung Carcinoma in Colombia (Geno1.1-CLICaP). Front Oncol. 2020. 10. p. 588932-588932. IF:6,244. (2).