Genomic analysis of ctx-m-group-1-producing extraintestinal pathogenic e. Coli (expec) from patients with urinary tract infections (uti) from colombia

Autores organización

• Maria Fernanda Mojica Medina

  Autor

• 

  Juan Carlos Garcia Betancur

  Autor

  betancurjuan@unbosque.edu.co

• 

  Christian Jose Pallares Gutierrez

  Autor

  cpallares@unbosque.edu.co

• 

  Maria Virginia Villegas Botero

  Autor

  mvvillegas@unbosque.edu.co

Autores

• Cadena E.D.L.
• Castillo N
• Correa A
• Appel TM

Grupos de investigación

• Grupo de Investigación en Resistencia Antimicrobiana y Epidemiología Hospitalaria RAEH

  

  Investigador

  Maria Virginia Villegas Botero

  mvvillegas@unbosque.edu.co

Resumen

Background: The dissemination of the uropathogenic O25b-ST131 Escherichia coli clone constitutes a threat to public health. We aimed to determine the circulation of E. coli strains belonging to O25b:H4-B2-ST131 and the H30-Rx epidemic subclone causing hospital and community-acquired urinary tract infections (UTI) in Colombia. Methods: Twenty-six nonduplicate, CTX-M group-1-producing isolates causing UTI in the hospital and community were selected for this study. Results: Twenty-two E. coli isolates harboring CTX-M-15, one CTX-M-3, and three CTX-M-55 were identified. Multilocus Sequence Typing (MLST) showed a variety of sequence types (STs), among which, ST131, ST405, and ST648 were reported as epidemic clones. All the E. coli ST131 sequences carried CTX-M-15, from which 80% belonged to the O25b:H4-B2 and H30-Rx pandemic subclones and were associated with virulence factors iss, iha, and sat. E. coli isolates (23/26) were resistant to ciprofloxacin and associated with amino acid substitutions in quinolone resistance-determining regions (QRDR). We detected two carbapenem-resistant E. coli isolates, one coproducing CTX-M-15, KPC-2, and NDM-1 while the other presented mutations in ompC. Additionally, one isolate harbored the gene mcr-1. Conclusions: Our study revealed the circulation of the E. coli ST131, O25b:H4-B2-H30-Rx subclone, harboring CTX-M-15, QRDR mutations, and other resistant genes. The association of the H30-Rx subclone with sepsis and rapid dissemination warrants attention from the public health and infections control. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

• bacterial protein; beta lactamase CTX M; cefepime; ceftriaxone; ciprofloxacin; ertapenem; fosfomycin; genomic DNA; piperacillin plus tazobactam; quinolone; virulence factor; amino acid substitution; antibiotic sensitivity; Article; bacterial gene; bacterial strain; bacterial virulence; bacterium isolate; broth dilution; carbapenem resistance; carbapenem resistant Escherichia coli; catB3 gene; cefotaxime resistance; ceftazidime resistance; cephalosporin resistance; emergency ward; Escherichia coli; extended spectrum beta lactamase producing Escherichia coli; fimH gene; fumC gene; gene; genome analysis; gyrA gene; hospitalization; human; intensive care unit; mcr 1 gene; mdfA gene; minimum inhibitory concentration; multilocus sequence typing; nonhuman; ompC gene; parC gene; prevalence; single nucleotide polymorphism; tetA gene; urinary tract infection; whole genome sequencing