Portal de investigación
Dynamics of blaKPC-2 Dissemination from Non-CG258 Klebsiella pneumoniae to Other Enterobacterales via IncN Plasmids in an Area of High Endemicity
Fecha de publicación:
Fecha Ahead of Print:
Autores organización
Autores
- Rada AM
- Agudelo C
- Capataz C
- Orozco N
- Dinh AQ
- Ríos R
- Diaz L
- Correa A
- Hanson BM
- Arias CA
- Restrepo E
Grupos de investigación
Resumen
Carbapenem-resistant Enterobacterales (CRE) pose a significant threat to global public health. The most important mechanism for carbapenem resistance is the production of carbapenemases. Klebsiella pneumoniae carbapenemase (KPC) represents one of the main carbapenemases worldwide. Complex mechanisms of blaKPC dissemination have been reported in Colombia, a country with a high endemicity of carbapenem resistance. Here, we characterized the dynamics of dissemination of blaKPC gene among CRE infecting and colonizing patients in three hospitals localized in a highly endemic area of Colombia (2013 and 2015). We identified the genomic characteristics of KPC-producing Enterobacterales recovered from patients infected/ colonized and reconstructed the dynamics of dissemination of blaKPC-2 using both short and long read sequencing. We found that spread of blaKPC-2 among Enterobacterales in the participating hospitals was due to intra- and interspecies horizontal gene transfer (HGT) mediated by promiscuous plasmids associated with transposable elements that was originated from a multispecies outbreak of KPC-producing Enterobacterales in a neonatal intensive care unit. The plasmids were detected in isolates recovered in other units within the same hospital and nearby hospitals. The gene “epidemic” was driven by IncN-pST15-type plasmids carrying a novel Tn4401b structure and non-Tn4401 elements (NTEKPC) in Klebsiella spp., Escherichia coli, Enterobacter spp., and Citrobacter spp. Of note, mcr-9 was found to coexist with blaKPC-2 in species of the Enterobacter cloacae complex. Our findings suggest that the main mechanism for dissemination of blaKPC-2 is HGT mediated by highly transferable plasmids among species of Enterobacterales in infected/colonized patients, presenting a major challenge for public health interventions in developing countries such as Colombia. Copyright © 2020 American Society for Microbiology. All Rights Reserved.
Copyright © 2020 American Society for Microbiology.
Datos de la publicación
- ISSN/ISSNe:
- 0066-4804, 1098-6596
- Tipo:
- Article
- Páginas:
- -
- DOI:
- 10.1128/AAC.01743-20
- Enlace a otro recurso:
- www.scopus.com
Antimicrobial Agents And Chemotherapy American Society for Microbiology
Citas Recibidas en Web of Science: 24
Citas Recibidas en Scopus: 35
Documentos
- No hay documentos
Filiaciones
Keywords
- Bacterial Proteins; beta-Lactamases; Carbapenems; Colombia; Humans; Infant, Newborn; Klebsiella Infections; Klebsiella pneumoniae; Plasmids; bacterial protein; beta lactamase; carbapenem derivative; Colombia; genetics; human; Klebsiella infection; Klebsiella pneumoniae; newborn; plasmid
Proyectos asociados
Caracterización de los mecanismos de resistencia en aislamientos clínicos de enterobacterias resistentes a ceftazidime/avibactam recolectados en 13 instituciones de salud entre 2016 y 2017.
Investigador Principal: MARIA FERNANDA MOJICA MEDINA
PCI-2019-10808 . 2020
Fortalecimiento de un laboratorio para el diagnóstico molecular de enfermedades infecciosas y la atención de problemáticas asociadas con agentes biológicos de alto riesgo para la salud humana en Bogotá
Investigador Principal: JAIME EDUARDO CASTELLANOS PARRA
UEB-2020-571 . 2020
Creating a Latin America network for clinical training and support of pharmacists and hospital financial decision makers involved in ASPs
Investigador Principal: MARIA VIRGINIA VILLEGAS BOTERO
UEB-2020-585 . 2020
Caracterización Molecular de Aislamientos Resistentes a Ceftazidime Avibactam en Enterobacterales y Pseudomonas aeruginosa recolectados en cinco Países de América Latina. Molecular mechanisms leading to ceftazidime-avibactam resistance in Enterobacterales and Pseudomonas aeruginosa collected from five Latin American countries
Investigador Principal: MARIA VIRGINIA VILLEGAS BOTERO
UEB-2020-586 . 2020
Citar la publicación
Rada AM,De La Cadena E,Agudelo C,Capataz C,Orozco N,Pallares C,Dinh AQ,Panesso D,Ríos R,Diaz L,Correa A,Hanson BM,Villegas MV,Arias CA,Restrepo E. Dynamics of blaKPC-2 Dissemination from Non-CG258 Klebsiella pneumoniae to Other Enterobacterales via IncN Plasmids in an Area of High Endemicity. Antimicrob Agents Chemother. 2020. 64. (12):e01743-20. IF:5,191. (1).