Prevalence of dengue antibodies in healthy children and adults in different Colombian endemic areas

Autores organización

• 

  Myriam Lucia Velandia Romero

  Autor

• Carolina Coronel Ruiz

  Autor

• Maria Angelica Calderon Pelaez

  Autor

• Alexandra Porras Ramirez

  Autor

• 

  Jaime Eduardo Castellanos Parra

  Autor

Autores

• Castro-Bonilla L
• Camacho-Ortega S
• Castellanos A
• Olano VA
• Arevalo-Herrera M
• Villamil-Gómez W

Grupos de investigación

• Grupo de Inmunología celular y molecular Universidad El Bosque (InmuBo)

  Investigador

  Maria Consuelo Romero Sanchez

• Grupo de Virología

  

  Investigador

  Jaime Eduardo Castellanos Parra

Resumen

Objectives: Colombia is a dengue hyperendemic country; however, the prevalence of antibodies against dengue in the general population including the inhabitants of rural areas is unknown. This study aimed to determine the prevalence of dengue IgM and IgG antibodies in healthy children and adults in urban and rural areas of seven different endemic regions in Colombia between 2013 and 2015. Design or method: Blood samples from healthy volunteers (1,318) were processed by serology (by indirect IgG and capture IgM and IgG ELISA) and molecular tests to detect viral RNA and circulating serotypes. Results: The seroprevalence of IgG for dengue were 85% in children and over 90% for adults. In addition to the high IgM positive rate (14.9%) and secondary recent infection marker rate (capture IgG, 16%), 8.4% of the healthy volunteers were positive for dengue virus (DENV) RNA. Conclusion: This study confirmed the broad and permanent circulation of DENV in Colombia and the high rates of infection and reinfection suffered by its inhabitants. This information can be used by the health authorities to strengthen vector control and vaccine policies and review the algorithms of diagnosis and disease management in children and adults. © 2019 The Author(s)

Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.

Keywords

• Adolescent; Adult; Aged; Aged, 80 and over; Antibodies, Viral; Child; Child, Preschool; Coinfection; Colombia; Dengue; Dengue Virus; Endemic Diseases; Female; Humans; Immunoglobulin G; Immunoglobulin M; Male; Middle Aged; RNA, Viral; Seroepidemiologic Studies; Serogroup; Young Adult; immunoglobulin G; immunoglobulin M; virus RNA; immunoglobulin G; immunoglobulin M; virus antibody; virus RNA; adolescent; adult; aged; antibody blood level; Article; blood sampling; child; Colombia; Colombian; controlled study; dengue; Dengue virus; enzyme linked immunosorbent assay; female; human; major clinical study; male; primary infection; reinfection; rural area; secondary infection; seroprevalence; urban area; blood; dengue; endemic disease; genetics; immunology; middle aged; mixed infection; preschool child; seroepidemiology; serotype; very elderly; young adult