Portal de investigación
Evaluation of Allplex™ Entero-DR assay for detection of antimicrobial resistance determinants from bacterial cultures
Fecha de publicación:
Fecha Ahead of Print:
Autores organización
Autores
- Correa A
- Appel TM
Grupos de investigación
Resumen
Objective: To evaluate the sensitivity and specificity of the Allplex™ Entero-DR, a quantitative PCR-based method, for the detection of ß-lactamase-encoding genes and vancomycin-resistance determinants in 156 previously characterized Gram-negative bacilli and Enterococcus spp. from bacterial cultures. Result: The method had 100% sensitivity and between 92 and 100% of specificity for identifying bla KPC, bla VIM, bla IMP, bla NDM, bla OXA-48-like, bla CTX-M and vanA. In nine isolates, unspecific amplifications were detected. The Ct of these false positives was above 33. The Ct of the correctly identified bla and van genes did not surpass 28 and 30, respectively. None of the clinical isolates included as negative controls yielded any amplification. Therefore, the Allplex™ Entero-DR assay is a highly accurate test for the detection of important antibiotic resistance determinants. With this assay, reliable results can be obtained within 3 h. However, according to our data, samples with Ct values greater than 33 should be considered with caution. © 2020 The Author(s).
Datos de la publicación
- ISSN/ISSNe:
- 1756-0500, 1756-0500
- Tipo:
- Article
- Páginas:
- 154-154
- Enlace a otro recurso:
- www.scopus.com
Bmc Research Notes BioMed Central Ltd
Citas Recibidas en Web of Science: 3
Citas Recibidas en Scopus: 4
Documentos
- No hay documentos
Filiaciones
Keywords
- Anti-Bacterial Agents; Bacterial Proteins; beta-Lactamases; Carbapenems; Carbon-Oxygen Ligases; Drug Resistance, Bacterial; Enterococcus; Gram-Negative Bacteria; Microbial Sensitivity Tests; Real-Time Polymerase Chain Reaction; Vancomycin; antiinfective agent; bacterial protein; beta lactamase; carbapenem derivative; ligase; VanA ligase, Bacteria; vancomycin; antibiotic resistance; drug effect; Enterococcus; genetics; Gram negative bacterium; isolation and purification; microbial sensitivity test; real time polymerase chain reaction
Proyectos asociados
Caracterización de los mecanismos de resistencia en aislamientos clínicos de enterobacterias resistentes a ceftazidime/avibactam recolectados en 13 instituciones de salud entre 2016 y 2017.
Investigador Principal: MARIA FERNANDA MOJICA MEDINA
PCI-2019-10808 . 2020
Citar la publicación
Mojica MF,De La Cadena E,Correa A,Appel TM,Pallares CJ,Villegas MV. Evaluation of Allplex™ Entero-DR assay for detection of antimicrobial resistance determinants from bacterial cultures. BMC Res Notes. 2020. 13. (1):p. 154-154.