Comparative Analysis of the Biosimilar and Innovative G-CSF Modulated Pathways on Umbilical Cord Blood–Derived Mononuclear Cells

Autores organización

• 

  Sandra Janneth Perdomo Lara

  Autor

Autores

• Avila-Portillo LM
• Aristizabal F
• Riveros A
• Ospino B
• Avila JP
• Butti M
• Abba MC

Grupos de investigación

• Grupo de Inmunología celular y molecular Universidad El Bosque (InmuBo)

  Investigador

  Maria Consuelo Romero Sanchez

Resumen

Biosimilars of granulocyte colony-stimulating factor (G-CSF) have been routinely introduced into clinical practice. However, not functional genomics characterization has been performed yet in comparison with the innovator G-CSF. This study aimed to evaluate the transcriptomic changes in an in vitro model of umbilical cord blood cells (UBC) exposed to G-CSF for the identification of their modulated pathways. Umbilical cord blood cells–derived mononuclear cells (MNCs) were treated with biosimilar and innovator G-CSF for further gene expression profiling analysis using a microarray-based platform. Comparative analysis of biosimilar and innovator G-CSF gene expression signatures allowed us to identify the most commonly modulated pathways by both drugs. In brief, we observed predominantly upmodulation of transcripts related to PI3K-Akt, NF-kappaB, and tumor necrosis factor (TNF) signaling pathways as well as transcripts related to negative regulation of apoptotic process among others. In addition, hematopoietic colony-forming cell assays corroborate the G-CSF phenotypic effects over UBC-derived MNCs. In conclusion, our study suggests that G-CSF impacts UBC-derived cells through the modulation of several signaling pathways associated with cell survival, migration, and proliferation. The concordance observed between biosimilar and innovator G-CSF emphasizes their similarity in regards to their specificity and biological responses. © The Author(s) 2020.

© The Author(s) 2020.

Keywords

• biosimilar agent; granulocyte colony stimulating factor; immunoglobulin enhancer binding protein; tumor necrosis factor; apoptosis; Article; cell migration; cell proliferation; cell survival; colony forming cell; comparative study; controlled study; gene expression; hematopoietic cell; human; human cell; microarray analysis; mononuclear cell; phenotype; Pi3K/Akt signaling; transcription regulation; transcriptomics; umbilical cord blood cell