Effect of Vitamins D and e on the Proliferation, Viability, and Differentiation of Human Dental Pulp Stem Cells: An in Vitro Study

Fecha de publicación: 01/01/2020 Fecha Ahead of Print: 01/07/2020

Autores organización

Autores

Bayona A

Grupos de investigación

Grupo de Virología

Investigador

Jaime Eduardo Castellanos Parra
Unidad de Manejo Integral de Malformaciones Craneofaciales UMIMC

Investigador

Maria Clara González Carrera

Resumen

Introduction. The aim of the present study was to determine the effects of vitamins D and E on the proliferation, morphology, and differentiation of human dental pulp stem cells (hDPSCs). Methods. In this in vitro experimental study, hDPSCs were isolated, characterized, and treated with vitamins D and E, individually and in combination, utilizing different doses and treatment periods. Changes in morphology and cell proliferation were evaluated using light microscopy and the resazurin assay, respectively. Osteoblast differentiation was evaluated with alizarin red S staining and expression of RUNX2, Osterix, and Osteocalcin genes using real-time RT-PCR. Results. Compared with untreated cells, the number of cells significantly reduced following treatment with vitamin D (49%), vitamin E (35%), and vitamins D + E (61%) after 144 h. Compared with cell cultures treated with individual vitamins, cells treated with vitamins D + E demonstrated decreased cell confluence, with more extensive and flatter cytoplasm that initiated the formation of a significantly large number of calcified nodules after 7 days of treatment. After 14 days, treatment with vitamins D, E, and D + E increased the transcription of RUNX2, Osterix, and Osteocalcin genes. Conclusions. Vitamins D and E induced osteoblastic differentiation of hDPSCs, as evidenced by the decrease in cell proliferation, morphological changes, and the formation of calcified nodules, increasing the expression of differentiation genes. Concurrent treatment with vitamins D + E induces a synergistic effect in differentiation toward an osteoblastic lineage. © 2020 Lina M. Escobar et al.

Datos de la publicación

ISSN/ISSNe:: 1687-8728, 1687-8736
Tipo:: Article
Páginas:: 8860840-8860840
DOI:: 10.1155/2020/8860840
Enlace a otro recurso:: www.scopus.com

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Métricas

Filiaciones

Escobar LM:: Universidad. Unidad de Manejo Integral de Malformaciones Craneofaciales (UMIMC), Facultad de Odontología, Universidad El Bosque, Bogotá, Colombia

Universidad. Posgrado de Ortodoncia, Facultad de Odontología, Universidad El Bosque, Bogotá, Colombia
Bendahan Z:: Universidad. Unidad de Manejo Integral de Malformaciones Craneofaciales (UMIMC), Facultad de Odontología, Universidad El Bosque, Bogotá, Colombia
Bayona A:: Universidad. Posgrado de Ortodoncia, Facultad de Odontología, Universidad El Bosque, Bogotá, Colombia
Castellanos JE:: Universidad. Vicerrectoría de Investigaciones, Universidad El Bosque, Bogotá, Colombia
González M.-C.:: Universidad. Unidad de Manejo Integral de Malformaciones Craneofaciales (UMIMC), Facultad de Odontología, Universidad El Bosque, Bogotá, Colombia

Keywords

alizarin red s; alpha tocopherol; osteocalcin; transcription factor osterix; transcription factor RUNX2; vitamin D; adult; Article; cell differentiation; cell isolation; cell proliferation; cell structure; cell viability; controlled study; cytoplasm; dental pulp stem cell; drug effect; human; human cell; in vitro study; microscopy; osteoblast; real time reverse transcription polymerase chain reaction; resazurin assay; synergistic effect