Comparison of Cell Viability and Chemical Composition of Six Latest Generation Orthodontic Wires

Autores organización

• Lina Maria Escobar Marquez

  Autor

• Jaime Rodrigo Rivera Barrero

  Autor

• David Augusto Diaz Baez

  Autor

• 

  Ingrid Isabel Mora Diaz

  Autor

• 

  Gloria Ines Lafaurie Villamil

  Autor

Autores

• Arbelaez, E
• Torres, LF
• Villafañe, A
• Tanaka, M

Grupos de investigación

• Unidad de Investigación Básica Oral UIBO

  

  Investigador

  Gloria Ines Lafaurie Villamil

• Unidad de Manejo Integral de Malformaciones Craneofaciales UMIMC

  

  Investigador

  Maria Clara González Carrera

Resumen

Orthodontic wires are made of alloys containing different metals, including nickel. It is important to evaluate their biocompatibility prior to use, owing to their long-term use in patients. This in vitro study compared the cytotoxicity and chemical composition of six latest orthodontic wires: Fantasia (R), Tanzo (R), FLI (R), NT3 (R), DuoForce (R), and Gummetal (R). The before-use group consisted of wires that were not used in the mouth, and the after-use group consisted of wires that were used in the mouth for two months. The wires were placed in contact with human gingival fibroblasts (HGF) for 72 h, and cytotoxicity was determined using the resazurin test. The chemical composition and surface characterisation were evaluated by spectrometry and scanning electron microscopy. The groups were compared using ANOVA and Kruskal-Wallis test. Only the FLI (R) wires produced a 36% reduction in HGF viability (p<0.05) and presented greater irregularities and loss of polymer structure. After-use wires showed a significant reduction in the percentage of nickel and the appearance of new elements (oxygen and carbon). Therefore, it can be concluded that no toxic ion release was noticed in this study. Rhodium-coated wires were more stable than PTFE-coated wires, and only the FLI (R) wires showed a slight cytotoxic effect.

Keywords

• nickel; oxygen; polymer; rhodium; adult; Article; cell viability; chemical composition; chemical structure; comparative study; controlled study; cytotoxicity; fibroblast; human; human cell; in vitro study; reduction (chemistry); scanning electron microscopy; spectroscopy; surface property