Multilocus genotyping of Giardia intestinalis in pet dogs of Medellin Colombia

Autores organización

• 

  Liliana Morales De La Pava

  Autor

• Jacqueline Chaparro Olaya

  Autor

Autores

• Hernández, PC
• López-Osorio, S
• López-Arias, A
• Chaparro-Gutiérrez, JJ

Grupos de investigación

• Laboratorio de Parasitología Molecular

  Investigador

  Jacqueline Chaparro Olaya

Resumen

According to a few parasitological and epidemiological studies, Giardia is the most prevalent parasitic infection among pet dogs in the city of Medellin, the second-largest city in Colombia. This study determined the assemblages of Giardia in the fecal samples of dogs obtained from 18 veterinary centers of Medellin. One hundred fecal samples of dogs diagnosed with Giardia using microscopy were analyzed via nested polymerase chain reaction (PCR) using three genes (gdh, bg, and tpi). The PCR products were purified and sequenced, and phylogenetic analyses were conducted using the maximum likelihood algorithm of the three loci. From the 100 samples analyzed, 47 were Giardia-positive via PCR. Genotypes C and D were detected in six samples, neither of which were associated with human infection. However, the zoonotic potential of Giardia cannot be ruled out because of the small number of samples that could be sequenced for assemblage assignation.

Copyright © 2020 Elsevier B.V. All rights reserved.

Keywords

• Giardia intestinalis; Genetic assemblages; Pet dogs; Multilocus genotyping; Colombia