First Report and Comparative Genomics Analysis of a bla_OXA-244-Harboring Escherichia coli Isolate Recovered in the American Continent

Autores organización

• 

  Deisy Julieth Abril Riaño

  Autor

• 

  Zayda Lorena Corredor Rozo

  Autor

• 

  Javier Antonio Escobar Perez

  Autor

Autores

• Moya, IGB
• Marquez-Ortiz, RA
• Montero, DFJ
• Molina, IT
• Gómez, NV

Grupos de investigación

• Laboratorio de Genética Molecular Bacteriana LGMB

  

  Investigador

  Javier Antonio Escobar Perez

Resumen

The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here, we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harboring the bla(OXA-244) gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study, and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other bla(OXA-244)-harboring Escherichia coli strains was performed. The 28Eco12 isolate belonged to sequence type (ST) 38, and its genome was composed of two molecules, a chromosome of 5,343,367 bp and a plasmid of 92,027 bp, which belonged to the incompatibility group IncY and did not harbor resistance genes. The bla(OXA-244) gene was chromosomally encoded and mobilized by an ISR1-related Tn6237 composite transposon. Notably, this transposon was inserted and located within a new genomic island. To our knowledge, this is the first report of a bla(OXA-244)-harboring Escherichia coli isolate in America. Our results suggest that the introduction of the OXA-244-producing E. coli isolate was through clonal expansion of the ST38 pandemic clone. Other isolates producing OXA-244 could be circulating silently in America.

Keywords

• bla(OXA-244); Escherichia coli; carbapenems; resistance; Colombia