Genome plasticity favours double chromosomal Tn4401b-bla_KPC-2 transposon insertion in the Pseudomonas aeruginosa ST235 clone

Fecha de publicación: 20/02/2019 Fecha Ahead of Print: 20/02/2019

Autores organización

Autores

Marquez-Ortiz, RA
Moncayo-Ortiz, JI
Escobar, NMO
Reyes, N
Tovar, C
Sánchez, HF
Guaca-González, YM
Llanos-Uribe, CE
Gómez, NV

Grupos de investigación

Grupo de Virología

Investigador

Jaime Eduardo Castellanos Parra
Laboratorio de Genética Molecular Bacteriana LGMB

Investigador

Javier Antonio Escobar Perez
Unidad de Genética y Resistencia Antimicrobiana UGRA

Resumen

BackgroundPseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms involved in resistance dissemination in bla(KPC-2)-positive P. aeruginosa ST235 in Colombia.ResultsIn a prospective surveillance study of infections in adult patients attended in five ICUs in five distant cities in Colombia, 58 isolates of P. aeruginosa were recovered, of which, 27 (46.6%) were resistant to carbapenems. The molecular analysis showed that 6 (22.2%) and 4 (14.8%) isolates harboured the bla(VIM) and bla(KPC-2) genes, respectively. The four bla(KPC-2)-positive isolates showed a similar PFGE pulsotype and belonged to ST235. Complete genome sequencing of a representative ST235 isolate shows a unique chromosomal contig of 7097.241bp with eight different resistance genes identified and five transposons: a Tn6162-like with ant(2)-Ia, two Tn402-like with ant(3)-Ia and bla(OXA-2) and two Tn4401b with bla(KPC-2). All transposons were inserted into the genomic islands. Interestingly, the two Tn4401b copies harbouring bla(KPC-2) were adjacently inserted into a new genomic island (PAGI-17) with traces of a replicative transposition process. This double insertion was probably driven by several structural changes within the chromosomal region containing PAGI-17 in the ST235 background.ConclusionThis is the first report of a double Tn4401b chromosomal insertion in P. aeruginosa, just within a new genomic island (PAGI-17). This finding indicates once again the great genomic plasticity of this microorganism.

Datos de la publicación

ISSN/ISSNe:: 1471-2180, 1471-2180
Tipo:: Article
Páginas:: 45-45
DOI:: 10.1186/s12866-019-1418-6

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Métricas

Filiaciones

Abril, D:: Universidad. Univ El Bosque, Bacterial Mol Genet Lab, Carrera 9 131A-02, Bogota, Colombia
Marquez-Ortiz, RA:: Universidad. Univ El Bosque, Bacterial Mol Genet Lab, Carrera 9 131A-02, Bogota, Colombia
Castro-Cardozo, B:: Universidad. Univ El Bosque, Bacterial Mol Genet Lab, Carrera 9 131A-02, Bogota, Colombia
Moncayo-Ortiz, JI:: Univ Tecnol Pereira, Fac Ciencias Salud, Grp Invest Enfermedades Infecciosas GRIENI, Pereira, Colombia
Escobar, NMO:: Hosp El Tunal ESE, Bogota, Colombia
Rozo, ZLC:: Universidad. Univ El Bosque, Bacterial Mol Genet Lab, Carrera 9 131A-02, Bogota, Colombia
Reyes, N:: Univ Cartagena, Grp Genet & Biol Mol, Cartagena, Colombia
Tovar, C:: Univ Sinu, Grp Invest Enfermedades Trop & Resistencia Bacter, Monteria, Colombia
Sánchez, HF:: Hosp Univ Dept Narino, Pasto, Colombia
Castellanos, J:: Univ Nacl Colombia, Grp Patogenesis Infecciosa, Bogota, Colombia
Guaca-González, YM:: Univ Tecnol Pereira, Fac Ciencias Salud, Grp Invest Enfermedades Infecciosas GRIENI, Pereira, Colombia
Llanos-Uribe, CE:: ESE Hosp Univ San Jorge, Pereira, Colombia
Gómez, NV:: Universidad. Univ El Bosque, Bacterial Mol Genet Lab, Carrera 9 131A-02, Bogota, Colombia

Univ Technol, Fac Sci, Inst I3, Sydney, NSW, Australia
Escobar-Pérez, J:: Universidad. Univ El Bosque, Bacterial Mol Genet Lab, Carrera 9 131A-02, Bogota, Colombia