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Anticuerpos policlonales contra la proteína recombinante NS3 del virus del dengue

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Resumen

Introduction: Dengue is a disease caused by one of four serotypes of the dengue virus (DENV) and is endemic in approximately 130 countries. The incidence of dengue has increased dramatically in recent decades, as well as the frequency and magnitude of outbreaks. Despite all efforts, there are no prophylactic or therapeutic treatments for the disease. Accordingly, research on the processes governing the DENV infection cycle is essential to develop vaccines or antiviral therapies. One of the most attractive DENV molecules to investigate is nonstructural protein 3 (NS3), which is essential for viral replication and a major immune target for infection. Objective: To produce antibodies to support future studies on NS3 and its cellular interactions with other proteins. Materials and methods: Two recombinant proteins of the helicase domain of DENV NS3 serotype 2 were expressed, and used to immunize mice and produce polyclonal antibodies. Results: The antibodies produced were useful in Western blot and immunofluorescence tests. We report an NS3 antibody that immunoprecipitates the viral protein and detects it in Western blot with no need to over-express it or use cell extracts with metabolic radiolabeling. Conclusion: The recombinant proteins expressed and the antibodies produced constitute valuable tools for studying DENV infectious processes involving NS3 and for evaluating tests designed to interfere with its functions.

Datos de la publicación

ISSN/ISSNe:
0120-4157, 2590-7379

Biomedica  Instituto Nacional de Salud

Tipo:
Article
Páginas:
131-140
PubMed:
28527257
Enlace a otro recurso:
www.scielo.org.co

Citas Recibidas en Scopus: 5

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Keywords

  • Animals; Antibodies, Viral; Blotting, Western; Dengue; Dengue Virus; Humans; Mice; RNA Helicases; Serine Endopeptidases; Viral Nonstructural Proteins; Virus Replication; NS3 protein, flavivirus; RNA helicase; serine proteinase; viral protein; virus antibody; animal; chemistry; dengue; Dengue virus; genetics; human; immunology; metabolism; mouse; physiology; virology; virus replication; Western blotting

Proyectos asociados

Análisis de la participación de los exosomas producidos por macrófagos en la activación endotelial y la dispersión del virus dengue-2

Investigador Principal: MYRIAM LUCIA VELANDIA ROMERO

UEB-2014-354 . 2015

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