Biopolymers of Polycaprolactone Loaded with Caffeic Acid and Trametes versicolor Extract Induced Proliferation in Human Coronary Artery Endothelial Cells and Inhibited Platelet Activity

Autores organización

• Diego Fernando Gualtero Escobar

  Autor

• Diana Marcela Buitrago Ramirez

  Autor

• 

  Gloria Ines Lafaurie Villamil

  Autor

Autores

• Pinzón-García A.D.
• Cely Veloza W.F.
• Figueroa-Ariza L.T.
• Torres-Morales S.
• Rodriguez-Navarrete J.D.
• Jimenez V.J.

Unidades de investigación

• Grupo de Inmunología celular y molecular Universidad El Bosque (InmuBo)

  Investigador

  Maria Consuelo Romero Sanchez

• Unidad de Investigación Básica Oral UIBO

  

  Investigador

  Gloria Ines Lafaurie Villamil

Resumen

In atherosclerosis, the proliferation and migration of endothelial and smooth muscle cells (SMCs) and platelet activation alter endothelial function. Naturally occurring substances, such as caffeic acid (CA) and Trametes versicolor extract (TvE), have medicinal properties and are traditionally used for their antiproliferative, antioxidant, and anti-inflammatory effects. Electrospun 5% and 8% polycaprolactone-loaded CA or TvE was developed as a delivery system. Cytocompatibility was evaluated using human coronary artery endothelial cells (HCAECs), coronary artery SMCs (CASMCs), and platelets. Three types of systems (µF-CA, µF-TvE, and µF-CA/TvE) were developed and microscopically characterized. Analysis with scanning electron microscopy showed multidirectional fibers with diameters of 2–4.5 µm. The µF systems were hydrophobic and low cellular adhesion. The viability of CASMCs decreased with microfibers of 8% PCL and high CA concentration. However, the viability of CASMCs and HCAECs improved with 5% PCL and low CA concentration. Treatment with µF-TvE and µF-CA/TvE increased cell viability. HCAEC proliferation was affected by µF-CA, but incorporating TvE improved it. Platelet viability was unaffected by any µF system, but µF-CA and µF-CA/TvE inhibited the activation and adhesion of platelets. The results suggest that microfibers loaded with CA and TvE play a dual role in modifying HCAEC proliferation and blocking human platelet activation and adhesion. These findings have the potential to mitigate the atherosclerotic process. © 2025 by the authors.

Keywords

• Biopolymers; Blood Platelets; Caffeic Acids; Cell Proliferation; Cell Survival; Cells, Cultured; Coronary Vessels; Endothelial Cells; Humans; Myocytes, Smooth Muscle; Plant Extracts; Platelet Activation; Polyesters; Trametes; biopolymer; caffeic acid; chitosan; endothelial leukocyte adhesion molecule 1; nanofiber; plant extract; platelet endothelial cell adhesion molecule 1; polycaprolactone; Trametes versicolor extract; unclassified drug; biopolymer; caffeic acid; caffeic acid derivative; plant extract; polycaprolactone; polyester; antiplatelet activity; Article; CASMC cell line; cell activation; cell adhesion; cell proliferation; cell viability; concentration (parameter); contact angle; controlled study; coronary artery endothelial cell; cytotoxicity; electrospinning; endothelium cell; human; human cell; hydrophobicity; scanning electron microscopy; smooth muscle cell; thrombocyte; thrombocyte activation; thrombocyte adhesion; thrombocyte function; Trametes versicolor; cell culture;