Osteoblastic differentiation and changes in the redox state in pulp stem cells by laser treatment

Autores organización

• 

  Lina María Vargas Escobar

  Autor

• Zita Carolina Bendahan Alvarez

  Autor

Autores

• Grajales M.
• Jaimes S.
• Baldión P.

Unidades de investigación

• Cuidado de la Salud y Calidad de Vida

  

  Investigador

  Miguel Antonio Sanchez Cardenas

• Salud y Sociedad UEB

  Investigador

  Olga Stella Diaz Usme

• Unidad de Manejo Integral de Malformaciones Craneofaciales UMIMC

  

  Investigador

  Maria Clara González Carrera

Resumen

The aim of this study was to determine the effect of low-level laser therapy (LLLT) on cell proliferation, mitochondrial membrane potential changes (??m), reactive oxygen species (ROS), and osteoblast differentiation of human dental pulp stem cells (hDPSCs). These cells were irradiated with 660- and 940-nm lasers for 5 s, 50 s, and 180 s. Cell proliferation was assessed using the resazurin assay, cell differentiation by RUNX2 and BMP2 expression, and the presence of calcification nodules using alizarin-red S staining. ROS was determined by the dichlorofluorescein-diacetate technique and changes in ??m by the tetramethylrhodamine-ester assay. Data were analyzed by a Student’s t-test and Mann–Whitney U test. The 940-nm wavelength for 5 and 50 s increased proliferation at 4 days postirradiation. After 8 days, a significant decrease in proliferation was observed in all groups. Calcification nodules were evident in all groups, with a greater staining intensity in cells treated with a 940-nm laser for 50 s, an effect that correlated with increased RUNX2 and BMP2 expression. ROS production and ?m increased independently of irradiation time. In conclusion, photobiomodulation (PBM) with LLLT induced morphological changes and reduced cell proliferation rate, which was associated with osteoblastic differentiation and increased ROS and ?m, independent of wavelength and time. © The Author(s) 2024.

Keywords

• Human dental pulp stem cells; Low-level laser therapy; Osteoblast differentiation; Photobiomodulation; Reactive oxygen species;Calcinosis; Cell Differentiation; Core Binding Factor Alpha 1 Subunit; Humans; Oxidation-Reduction; Reactive Oxygen Species; Stem Cells; bone morphogenetic protein 2; reactive oxygen metabolite; transcription factor RUNX2; reactive oxygen metabolite; transcription factor RUNX2; Article; cell differentiation; cell proliferation; cell proliferation rate; clinical article; controlled study; dental pulp stem cell; flow cytometry; fluorescence intensity; fluorescence microscopy; histogram; human; human cell; immunophenotyping; irradiation; laser therapy; low level laser therapy; mesenchyme cell; mitochondrial membrane potential; morphological trait; osteoblast; oxidation reduction state; oxidative phosphorylation; protein expression; resazurin assay; spectrofluorometry; calcinosis; cell differentiation; oxidation reduction reaction; stem cell