Dengue NS1 Antibodies Are Associated with Clearance of Viral Nonstructural Protein-1

Autores organización

• Alexandra Porras Ramirez

  Autor

• Franklin Alejandro Rico Mendoza

  Autor

Autores

• Premazzi Papa M.
• Mendoza-Torres E.
• Sun P.
• Encinales L.
• Goulet J.
• Defang G.
• Vihasi J.
• Cheng Y.
• Suchowiecki K.
• Rosales W.
• Amdur R.
• Herrera Gomez C.
• Nicholes S.
• Zuluaga I.
• Halstead L.
• Halstead S.
• Simon G.
• Porter K.
• Lynch R.M.
• Chang A.Y.

Unidades de investigación

• Esclerosis múltiple y otros trastornos neurológicos

  Investigador

  Jaime Toro Gomez

• Grupo de Investigación Medicina Materno Fetal y Ginecología - El Bosque MMFG-EB

  Investigador

  Ximena Carolina Romero Infante

• Medicina Comunitaria y Salud Colectiva

  Investigador principal

  Alexandra Porras Ramirez

• Saneamiento Ecológico, Salud y Medio Ambiente

  

  Investigador

  Diana Marcela Sarmiento Senior

Resumen

Background: Dengue vascular permeability syndrome is the primary cause of death in severe dengue infections. The protective versus potentially pathogenic role of dengue nonstructural protein-1 (NS1) antibodies are not well understood. The main goal of this analysis was to characterize the relationship between free NS1 concentration and NS1 antibody titers in primary and secondary dengue infection to better understand the presence and duration of NS1 antibody complexes in clinical dengue infections. Methods: Hospitalized participants with acute dengue infection were recruited from Northern Colombia between 2018 and 2020. Symptom assessment, including dengue signs and symptoms, chart review, and blood collection, was performed. Primary versus secondary dengue was assessed serologically. NS1 titers and anti-NS1 antibodies were measured daily. Results: Patients with secondary infection had higher antibody titers than those in primary infection, and there was a negative correlation between anti-NS1 antibody titer and NS1 protein. We demonstrate that in a subset of secondary infection, there were indeed NS1 antigen-Antibody complexes on the admission day during the febrile phase that were not detectable by the recovery phase. Furthermore, dengue infection status was associated with higher circulating sialidases. Discussion: The negative correlation between antibody and protein suggests that antibodies may play a role in clearing this viral protein. © 2024 The Author(s).

Keywords

• acid dissociation; antigen-Antibody complexes; dengue; neutralization; NS1 antibodies; sialidase 2;Adolescent; Adult; Antibodies, Viral; Antigen-Antibody Complex; Colombia; Dengue; Dengue Virus; Female; Humans; Male; Middle Aged; Viral Nonstructural Proteins; Young Adult; immunoglobulin G; immunoglobulin M; neutralizing antibody; nonstructural protein 1; sialidase; viral protein; viral nonstructural protein; virus antibody; adolescent; antibody titer; Article; blood sampling; child; clinical article; dengue; Dengue virus; disease severity; enzyme linked immunosorbent assay; female; human; male; polymerase chain reaction; real time polymerase chain reaction; RNA extraction; seroprevalence; serotyping; vaccination; virus neutralization; adult; antigen antibody complex; blood; Colombia; Dengue virus; epidemiology; immunology; middle aged; young adult