Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia

Autores organización

• 

  Myriam Lucia Velandia Romero

  Autor

• Carolina Coronel Ruiz

  Autor

• Maria Angelica Calderon Pelaez

  Autor

• 

  Jaime Eduardo Castellanos Parra

  Autor

Autores

• Olano V.A.
• Cabezas L.
• Matiz M.I.

Unidades de investigación

• Grupo de Virología

  

  Investigador

  Jaime Eduardo Castellanos Parra

Resumen

INTRODUCTION: There is a high incidence and prevalence of dengue in the department of Cundinamarca, and recently Aedes aegypti, the main vector of dengue virus (DENV), was detected in some of its rural areas. OBJECTIVE: To evaluate viral transovarial transmission in larvae and pupae collected in rural areas of the municipality of Anapoima, Cundinamarca. MATERIALS AND METHODS: Live larvae and pupae were collected from 53 homes and later they were taken to the laboratory in Anapoima, where they were classified, pooled and frozen. In Bogotá, they were homogenized, RNA was extracted with Trizol™, and RT-PCR and conventional PCR were performed. The amplified products were analyzed on 2% agarose gels. RESULTS: In 54.7% of the houses we found A. aegypti in immature stages, and DENV-1 was the most frequent serotype. However, the simultaneous presence of DENV 1 and 2, DENV 1 and 3, DENV 1 and 4, and DENV 1, 2 and 3 serotypes was detected in some pools. CONCLUSION: The results confirmed the natural vertical transmission of the virus in the rural area under study. These findings confirmed the vector capacity of A. aegypti, and partly explains the persistence of the virus in the region and the possibility of transmission by the vector during adulthood without having ingested infected blood. This situation increases the risk of DENV infection in Colombia and the need for prevention and control programs in all areas where the mosquito is present.

Keywords

• Aedes; Animals; Colombia; Dengue; Dengue Virus; Geography, Medical; Housing; Humans; Larva; Mosquito Vectors; Pupa; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral; Rural Health; Serotyping; virus RNA; Aedes; animal; classification; Colombia; comparative study; dengue; Dengue virus; genetics; housing; human; isolation and purification; larva; medical geography; mosquito vector; pupa; reverse transcription polymerase chain reaction; rural health; serotyping; transmission; virology