The frequency of hla-b27 in a Colombian population with signs of spondyloarthritis

Autores organización

• Maria Consuelo Romero Sanchez

  Autor

• Leidy Lorena Chila Moreno

  Autor

• Wilson Armando Bautista Molano

  Autor

• 

  Juliette De Avila Quiroga

  Autor

Autores

• Gómez A.
• María Consuelo Casas G.
• Briceño I.
• Rueda J.C.
• Londoño J.
• Valle-Oñate R.

Unidades de investigación

• Grupo de Inmunología celular y molecular Universidad El Bosque (InmuBo)

  Investigador

  Maria Consuelo Romero Sanchez

Resumen

Background: The strong association between HLA-B27 and spondyloarthritis (SpA) has demonstrated that typing the HLA-B27 antigen is a crucial step in diagnosis and aids in defining the progression and severity of disease. Objective: To describe the frequency of HLA-B27 in Colombian individuals with clinical manifestations associated with SpA. Materials and Methodology: We retrospectively analyzed 4109 HLA-B27 typing requests to the Hospital Militar Central and the Instituto de Referencia Andino from Colombian individuals with clinical signs suggestive of SpA between 2009 and 2012. We used basic digital cytometry followed by Polymerase Chain Reaction with sequence specific primers when confirmation was needed. We determined the frequency of HLA-B27 in the population and levels of association of HLA-B27 with SpA. Results: Our population included 1585 men (36.8%) and 2524 women (61.4%). The predominant age range was between 19 and 45 years (49.9%). The majority (95.4%) of the study population came from the Andean region and eastern plains. The most frequent clinical manifestations were peripheral. Only a small fraction (12.1%) of the 4109 subjects was HLA-B27 positive. Of those, 56.9% were male, and 54.7% were between 19 and 45 years old. In contrast, when rheumatologists referred the HLA B27, 64% were found to be positive. Conclusion: The frequency of the HLA-B27 allele in individuals with clinical signs suggestive of SpA was low, in accordance with the lower prevalence found in Colombian patients diagnosed with SpA compared to American and European population. © 2018 Bentham Science Publishers.

Keywords

• Adult; Colombia; Female; Gene Frequency; Genetic Predisposition to Disease; HLA-B27 Antigen; Humans; Male; Middle Aged; Molecular Epidemiology; Phenotype; Prevalence; Retrospective Studies; Spondylarthritis; Young Adult; HLA B27 antigen; HLA B27 antigen; adult; Article; Colombia; cytometry; disease association; disease severity; female; flow cytometry; gene frequency; human; major clinical study; male; middle aged; observational study; polymerase chain reaction; prevalence; priority journal; retrospective study; rheumatologist; sequence analysis; spondylarthritis; genetic predisposition; genetics; immunology; molecular epidemiology; phenotype; spondylarthritis; young adult