Factors associated with dengue virus infection and reinfection in asymptomatic children in two Colombian municipalities

Autores organización

• Carolina Coronel Ruiz

  Autor

• 

  Jaime Eduardo Castellanos Parra

  Autor

• Alexandra Porras Ramirez

  Autor

• 

  Myriam Lucia Velandia Romero

  Autor

Autores

• Castro-Bonilla L.
• Parra-Alvarez S.

Unidades de investigación

• Esclerosis múltiple y otros trastornos neurológicos

  Investigador

  Jaime Toro Gomez

• Grupo de Investigación Medicina Materno Fetal y Ginecología - El Bosque MMFG-EB

  Investigador

  Ximena Carolina Romero Infante

• Grupo de Virología

  

  Investigador

  Jaime Eduardo Castellanos Parra

• Medicina Comunitaria y Salud Colectiva

  Investigador principal

  Alexandra Porras Ramirez

• Saneamiento Ecológico, Salud y Medio Ambiente

  

  Investigador

  Diana Marcela Sarmiento Senior

Resumen

Dengue is the most important arbovirosis in the world. In this study, we assessed the knowledge, attitudes, and practices (KAP) regarding dengue in parents from two small Colombian municipalities in the Cundinamarca Province. Parents and their healthy children from 4 to 14 years of age were included in some public elementary schools. After a medical examination, blood samples were taken for diagnosis of dengue using enzyme-linked immunosorbent assays (capture immunoglobulin M and capture immunoglobulin G [IgG], indirect IgG and detection non-structural viral protein 1) and detection of viral RNA by reverse transcription polymerase chain reaction. In addition, a KAP survey was applied to the children’s parents or tutors. The indirect IgG test determined that of the 347 examined children, 87.9% had a previous infection with the dengue virus (DENV), 12.7% of them were positive for viral RNA (asymptomatic infection), and 32.0% presented reinfections. Risk factors evaluation showed that children aged 8 years and older living in the municipalities for more than 7 years were more likely to be infected or reinfected by DENV. In the same way, poor nutrition, lack of water supply, sewer service, or waste disposal services could raise the likelihood of dengue infections. The surveys indicated that parents have unhealthy practices and a low knowledge about the transmission of the disease, which could result in an increase of mosquito breeding sites, allowing sustained dengue transmission. Copyright © 2018 by The American Society of Tropical Medicine and Hygiene.

Keywords

• Adolescent; Aedes; Animals; Antibodies, Viral; Asymptomatic Infections; Child; Child, Preschool; Cities; Colombia; Dengue; Dengue Virus; Drinking Water; Female; Health Knowledge, Attitudes, Practice; Humans; Immunoglobulin G; Male; Malnutrition; Mosquito Vectors; Parents; Recurrence; Risk Factors; RNA, Viral; Sanitation; Viral Nonstructural Proteins; Water Quality; immunoglobulin G; immunoglobulin M; viral protein; virus RNA; drinking water; immunoglobulin G; viral protein; virus antibody; virus RNA; adolescent; adult; aged; Article; asymptomatic infection; attitude to health; blood sampling; breeding; child; child nutrition; Colombia; controlled study; dengue; Dengue virus; disease association; environmental sanitation; enzyme linked immunosorbent assay; female; high risk population; human; major clinical study; male; medical examination; parental attitude; primary school; protein analysis; reinfection; reverse transcription polymerase chain reaction; sewer; virus transmission; water