A strategy for entomo-virological surveillance of yellow fever, dengue, zika, and chikungunya viruses in field-collected mosquitoes

Fecha de publicación: 01/01/2023 Fecha Ahead of Print: 01/09/2023

Autores organización

Autores

Olano V.A.

Grupos de investigación

Grupo de Virología

Investigador

Jaime Eduardo Castellanos Parra
Saneamiento Ecológico, Salud y Medio Ambiente

Investigador

Diana Marcela Sarmiento Senior

Resumen

Arboviruses transmitted by Culicidae insects are significant threats to human health, presenting dynamic transmission cycles and involving different vectors and hosts. The surveillance and characterization of the vectors involved in these cycles are crucial for understanding and preventing potential outbreaks. Therefore, we propose a strategy that we used for entomological surveillance of urban, rural, and sylvatic mosquitoes and to characterize natural infection by four major arboviruses. • Immature and adult mosquitoes were collected intra, peri and extradomicilie of urban and rural households, using different collection methodologies. • Mosquitoes were pooled or separated in head-thorax and abdomen, according to the species. • A multiplex nested RT-PCR (Reverse transcription polymerase chain reaction) method was used for the simultaneous detection of dengue virus (DENV), zika virus (ZIKV), chikungunya virus (CHIKV), and yellow fever virus (YFV).Overall, this strategy proved helpful for vectors surveillance at different ecosystems, as well as for implementing a low-cost molecular surveillance system that allows the early detection of potential outbreaks, and identify other potential vectors involved in viral transmission. © 2023 The Authors

Datos de la publicación

ISSN/ISSNe:: 2215-0161, 2215-0161
Tipo:: Article
Páginas:: -
DOI:: 10.1016/j.mex.2023.102356
Enlace a otro recurso:: www.scopus.com

Documentos

No hay documentos

Métricas

Filiaciones

Calderón-Peláez M.-A.:: Universidad. Vicerrectoría de Investigaciones, Grupo de Virología, Universidad El Bosque, Bogotá, 110121, Colombia
Mantilla-Granados J.S.:: Universidad. Vicerrectoría de Investigaciones, Saneamiento Ecológico, Salud y Medio Ambiente, Universidad El Bosque, Bogotá, 110121, Colombia
Velandia-Romero M.L.:: Universidad. Vicerrectoría de Investigaciones, Grupo de Virología, Universidad El Bosque, Bogotá, 110121, Colombia
Calvo E.:: Universidad. Vicerrectoría de Investigaciones, Grupo de Virología, Universidad El Bosque, Bogotá, 110121, Colombia
Castellanos J.E.:: Universidad. Vicerrectoría de Investigaciones, Grupo de Virología, Universidad El Bosque, Bogotá, 110121, Colombia
Olano V.A.:: Universidad. Vicerrectoría de Investigaciones, Saneamiento Ecológico, Salud y Medio Ambiente, Universidad El Bosque, Bogotá, 110121, Colombia

Keywords

virus RNA; adult; Arbovirus; Article; chikungunya; Chikungunya virus; controlled study; dengue; Dengue virus; household; human; mosquito; multiplex polymerase chain reaction; nonhuman; sample size; taxonomic identification; yellow fever; Yellow fever virus; Zika fever