The Interaction Effect of Anti-RgpA and Anti-PPAD Antibody Titers: An Indicator for Rheumatoid Arthritis Diagnosis

Fecha de publicación: 01/01/2023 Fecha Ahead of Print: 21/04/2023

Autores organización

• 

  Diana Marcela Castillo Perdomo

  Autor

• 

  Gloria Ines Lafaurie Villamil

  Autor

• Maria Consuelo Romero Sanchez

  Autor

• 

  Nathaly Andrea Delgadillo Salgado

  Autor

• Yormaris Castillo Romero

  Autor

• Wilson Armando Bautista Molano

  Autor

• 

  Jaime Eduardo Castellanos Parra

  Autor

Autores

• Pacheco-Tena C
• Bello-Gualtero JM
• Chalem-Choueka P

Grupos de investigación

• Grupo de Inmunología celular y molecular Universidad El Bosque (InmuBo)

  Investigador

  Maria Consuelo Romero Sanchez

• Grupo de Virología

  

  Investigador

  Jaime Eduardo Castellanos Parra

• Unidad de Investigación Básica Oral UIBO

  

  Investigador

  Gloria Ines Lafaurie Villamil

Resumen

Porphyromonas gingivalis secretes virulence factors like Arg-gingipains and peptidyl arginine deiminase (PPAD), that are associated with rheumatoid arthritis (RA) pathogenesis. However, there is no information regarding the antibody titers for these bacterial enzymes as systemic indicators or biomarkers in RA. In this cross-sectional study, 255 individuals were evaluated: 143 were diagnosed with RA, and 112 were without RA. Logistic regression models adjusted for age, sex, basal metabolic index, smoking, and periodontitis severity were used to evaluate the association of RA with rheumatoid factor (RF), anti-citrullinated protein antibodies (ACPAs), erythrocyte sedimentation rate, high sensitivity C-reactive protein, anti-RgpA, anti-PPAD, and double positive anti-RgpA/anti-PPAD. It was found that RF (odds ratio [OR] 10.6; 95% confidence interval [CI] 4.4–25), ACPAs (OR 13.7; 95% CI 5.1–35), and anti-RgpA/anti-PPAD double positivity (OR 6.63; 95% CI 1.61–27) were associated with RA diagnoses. Anti-RgpA was also associated with RA (OR 4.09; 95% CI 1.2–13.9). The combination of anti-RgpA/anti-PPAD showed a high specificity of 93.7% and 82.5% PPV in identifying individuals with RA. RgpA antibodies were associated with the periodontal inflammatory index in RA individuals (p < 0.05). The double positivity of the anti-RgpA/anti-PPAD antibodies enhanced the diagnosis of RA. Therefore, RgpA antibodies and anti-RgpA/anti-PPAD may be biomarkers for RA. © 2023 by the authors.

Keywords

• antibody; antirheumatic agent; biological marker; biological product; C reactive protein; cyclic citrullinated peptide antibody; gingipain R; protein arginine deiminase; rheumatoid factor; adult; antibody detection; antibody titer; Article; basal metabolic rate; bleeding on probing; controlled study; cross-sectional study; diagnostic test accuracy study; disease severity; erythrocyte sedimentation rate; female; human; major clinical study; male; middle aged; nonhuman; observational study; periodontal inflammatory index; periodontal pocket depth; periodontitis; plaque index; Porphyromonas gingivalis; predictive value; rheumatoid arthritis; scoring system; sensitivity and specificity; smoking; tooth plaque